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ecori restriction enzymes  (New England Biolabs)


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    Structured Review

    New England Biolabs ecori restriction enzymes
    Ecori Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1644 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nhei+hf+restriction+enzymes/NheI-HF/pm42036043-165-42-48
    Average 96 stars, based on 1644 article reviews
    ecori restriction enzymes - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Vaccines and pharmaceutical compositions against foot-and-mouth disease virus
    Article Snippet: .. Restriction digestions were performed on both the pTarget-GLuc vector and the Δ1D2A translational interrupter sequence using XmaI and NheI-HF restriction enzymes (New England Biolabs) per manufacturer's instructions. .. Both vector and insert were purified using a PCR purification kit (Qiagen).

    Article Title: Exploration of oncogenic cooperation between germline variation and somatic mutation in prostate cancer progression
    Article Snippet: .. The fragment was cloned upstream of the SV40 promoter into the pGL3 luciferase reporter vector (Promega) digested with KpnI-HF and NheI-HF restriction enzymes (New England Biolabs) using NEBuilder ® HiFi DNA Assembly (New England Biolabs). .. The assembled DNA was then transformed in NEB ® Stable Competent E. coli (New England Biolabs) using ampicillin for selection (Sigma-Aldrich) at 100 μg/mL added to LB Broth (Carl Roth) at 30 °C overnight.

    Article Title: Method for producing foot-and-mouth disease virus (FMDV) viral proteins utilizing a modified FMDV 3C protease
    Article Snippet: .. Restriction digestions were performed on both the pTarget-GLuc vector and the Δ1D2A translational interrupter sequence using XmaI and NheI-HF restriction enzymes (New England Biolabs) per manufacturer's instructions. .. Both vector and insert were purified using a PCR purification kit (Qiagen).

    Article Title: DNA vaccines against foot-and-mouth disease virus
    Article Snippet: .. Restriction digestions were performed on both the pTarget-GLuc vector and the Δ1D2A translational interrupter sequence using XmaI and NheI-HF restriction enzymes (New England Biolabs) per manufacturer's instructions. .. Both vector and insert were purified using a PCR purification kit (Qiagen).

    Sequencing:

    Article Title: Vaccines and pharmaceutical compositions against foot-and-mouth disease virus
    Article Snippet: .. Restriction digestions were performed on both the pTarget-GLuc vector and the Δ1D2A translational interrupter sequence using XmaI and NheI-HF restriction enzymes (New England Biolabs) per manufacturer's instructions. .. Both vector and insert were purified using a PCR purification kit (Qiagen).

    Article Title: Method for producing foot-and-mouth disease virus (FMDV) viral proteins utilizing a modified FMDV 3C protease
    Article Snippet: .. Restriction digestions were performed on both the pTarget-GLuc vector and the Δ1D2A translational interrupter sequence using XmaI and NheI-HF restriction enzymes (New England Biolabs) per manufacturer's instructions. .. Both vector and insert were purified using a PCR purification kit (Qiagen).

    Article Title: DNA vaccines against foot-and-mouth disease virus
    Article Snippet: .. Restriction digestions were performed on both the pTarget-GLuc vector and the Δ1D2A translational interrupter sequence using XmaI and NheI-HF restriction enzymes (New England Biolabs) per manufacturer's instructions. .. Both vector and insert were purified using a PCR purification kit (Qiagen).

    Clone Assay:

    Article Title: Exploration of oncogenic cooperation between germline variation and somatic mutation in prostate cancer progression
    Article Snippet: .. The fragment was cloned upstream of the SV40 promoter into the pGL3 luciferase reporter vector (Promega) digested with KpnI-HF and NheI-HF restriction enzymes (New England Biolabs) using NEBuilder ® HiFi DNA Assembly (New England Biolabs). .. The assembled DNA was then transformed in NEB ® Stable Competent E. coli (New England Biolabs) using ampicillin for selection (Sigma-Aldrich) at 100 μg/mL added to LB Broth (Carl Roth) at 30 °C overnight.

    Luciferase:

    Article Title: Exploration of oncogenic cooperation between germline variation and somatic mutation in prostate cancer progression
    Article Snippet: .. The fragment was cloned upstream of the SV40 promoter into the pGL3 luciferase reporter vector (Promega) digested with KpnI-HF and NheI-HF restriction enzymes (New England Biolabs) using NEBuilder ® HiFi DNA Assembly (New England Biolabs). .. The assembled DNA was then transformed in NEB ® Stable Competent E. coli (New England Biolabs) using ampicillin for selection (Sigma-Aldrich) at 100 μg/mL added to LB Broth (Carl Roth) at 30 °C overnight.

    Cloning:

    Article Title: In silico MS/MS prediction for peptidoglycan profiling uncovers novel anti-inflammatory peptidoglycan fragments of the gut microbiota
    Article Snippet: .. Cloning, overexpression, and purification of putative Bifidobacterium LTs The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S15), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs). .. Cloning, overexpression, and purification of putative Bifidobacterium LTs The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S15), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs).

    Article Title: Biochemical characterization of Bifidobacterium bifidum peptidoglycan dl -endopeptidase BbMep that generates NOD2 ligands
    Article Snippet: .. In addition, peak areas were also corrected for natural isotopic distribution and the number of PGN monomeric units.2 Cloning, overexpression, and purification of Bifidobacterium endopeptidases The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S9), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs). ..

    Over Expression:

    Article Title: In silico MS/MS prediction for peptidoglycan profiling uncovers novel anti-inflammatory peptidoglycan fragments of the gut microbiota
    Article Snippet: .. Cloning, overexpression, and purification of putative Bifidobacterium LTs The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S15), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs). .. Cloning, overexpression, and purification of putative Bifidobacterium LTs The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S15), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs).

    Article Title: Biochemical characterization of Bifidobacterium bifidum peptidoglycan dl -endopeptidase BbMep that generates NOD2 ligands
    Article Snippet: .. In addition, peak areas were also corrected for natural isotopic distribution and the number of PGN monomeric units.2 Cloning, overexpression, and purification of Bifidobacterium endopeptidases The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S9), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs). ..

    Purification:

    Article Title: In silico MS/MS prediction for peptidoglycan profiling uncovers novel anti-inflammatory peptidoglycan fragments of the gut microbiota
    Article Snippet: .. Cloning, overexpression, and purification of putative Bifidobacterium LTs The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S15), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs). .. Cloning, overexpression, and purification of putative Bifidobacterium LTs The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S15), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs).

    Article Title: Biochemical characterization of Bifidobacterium bifidum peptidoglycan dl -endopeptidase BbMep that generates NOD2 ligands
    Article Snippet: .. In addition, peak areas were also corrected for natural isotopic distribution and the number of PGN monomeric units.2 Cloning, overexpression, and purification of Bifidobacterium endopeptidases The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S9), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs). ..

    Amplification:

    Article Title: In silico MS/MS prediction for peptidoglycan profiling uncovers novel anti-inflammatory peptidoglycan fragments of the gut microbiota
    Article Snippet: .. Cloning, overexpression, and purification of putative Bifidobacterium LTs The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S15), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs). .. Cloning, overexpression, and purification of putative Bifidobacterium LTs The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S15), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs).

    Article Title: Biochemical characterization of Bifidobacterium bifidum peptidoglycan dl -endopeptidase BbMep that generates NOD2 ligands
    Article Snippet: .. In addition, peak areas were also corrected for natural isotopic distribution and the number of PGN monomeric units.2 Cloning, overexpression, and purification of Bifidobacterium endopeptidases The gene sequences encoding for the respective proteins lacking the transmembrane helix were amplified from genomic DNA with suitable primers (Table S9), and inserted into the pET28a(+) vector with XhoI and NheI-HF restriction enzymes (New England Biolabs). ..



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